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hy 15979 pki medchemexpress  (MedChemExpress)


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    MedChemExpress hy 15979 pki medchemexpress
    Hy 15979 Pki Medchemexpress, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 228 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pki+medchemexpress/H-89/pm36863348-199-104-106
    Average 97 stars, based on 228 article reviews
    hy 15979 pki medchemexpress - by Bioz Stars, 2026-10
    97/100 stars

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    Article Title: Gut microbiota-mediated secondary bile acids regulate dendritic cells to attenuate autoimmune uveitis through TGR5 signaling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse CD4 APC eBioscience Cat# 17-0042-81; RRID:AB_469322 Anti-mouse IL-17a PE eBioscience Cat# 12-7177-81; RRID:AB_763582 Anti-mouse IFN-g PE-cy7 eBioscience Cat# 25-7311-82; RRID:AB_469680 Anti-mouse CD25 PE eBioscience Cat# 12-0251-81; RRID:AB_465606 Anti-mouse Foxp3 PE-cy7 eBioscience Cat# 25-5773-80; RRID:AB_891554 Anti-mouse CD11c APC BioLegend Cat# 117310; RRID:AB_313779 Anti-mouse CD40 FITC BioLegend Cat# 124607; RRID:AB_1134090 Anti-mouse MHCII PE eBioscience Cat# 12-5321-81; RRID:AB_465927 Anti-mouse CD80 PE eBioscience Cat# 12-0801-81; RRID:AB_465751 Anti-mouse CD86 FITC eBioscience Cat# 11-0860-82; RRID:AB_465145 Anti-human CD80 PE BioLegend Cat# 375410; RRID:AB_2890820 Anti-human CD86 PE-cy7 BioLegend Cat# 374209; RRID:AB_2728391 Anti-human HLA-DR PE-cy5.5 BioLegend Cat# 307629; RRID:AB_893575 Anti-human CD40 FITC BioLegend Cat# 334305; RRID:AB_1186056 Mouse CD4 microbeads Miltenyi Biotec Cat# 130-049-201; RRID:AB_2722753 Human CD14 microbeads Miltenyi Biotec Cat# 130-097-052; RRID:AB_2665482 Anti-TGR5 Abcam Cat# ab72608; RRID:AB_2112165 Anti-P65 Abcam Cat# ab16502; RRID:AB_443394 Anti-NF-kB p65 (Phospho Ser536) CST Cat# 3033T; RRID:AB_331284 Anti-Ikba Abcam Cat# ab32518; RRID:AB_733068 Anti-Ikba (phospho S36) Abcam Cat# ab133462; RRID:AB_2801653 Anti-CREB Abcam Cat# ab32515; RRID:AB_2292301 Anti-CREB(phosphor S133) Abcam Cat# ab32096; RRID:AB_731734 Anti-b-actin Abcam Cat# ab8227; RRID:AB_2305186 Experimental models: Organisms/strains C57BL/6j mice Jachson Lab N/A TGR5 / mice Viewsolid biotech N/A Bacterial and virus strains Clostridium scindens ATCC ATCC35704 Chemicals, peptides, and recombinant proteins IRBP651-670 Sangon Biotech N/A Mycobacterium tuberculosis strain H37Ra BD Cat# 231141 Bordetella pertussis toxin Sigma Cat# P7208 CFA Sigma Cat# F5881 QIAamp Fast DNA Stool Mini Kit QIAGEN Cat# 51604 Neomycin Sigma Cat# N6386 Ampicillin Sigma Cat# A5354 Metronidazole Sigma Cat# 16677 Vancomycin Sigma Cat# V2002 TRIzol Reagent Invitrogen Cat# 15596026 PrimeScript RT reagent Kit Takara Cat# DRR047A iTaq Universal SYBR Green Supermix BIO-RAD Cat# 1725124 (Continued on next page) e1 Cell Reports 36, 109726, September 21, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER mouse GM-CSF R&D Cat# 415-ML-50 mouse IL-4 R&D Cat# 404-ML-50 Lipopolysaccharide Sigma Cat# L2880 DCA Sigma Cat# L6250 INT-777 MedChemExpress Cat# HY-15677 Forskolin MedChemExpress Cat# HY-15371 IBMX MedChemExpress Cat# HY-12318 KH7 MedChemExpress Cat# HY-103194 MDL12330A Sigma Cat# 444200 PKI MedChemExpress Cat# HY-P0222 H89 MedChemExpress Cat# HY-15979 Foxp3 / Transcription Factor Staining Buffer Set Kit Invitrogen Cat# 00-5523-00 PMA Sigma Cat# P1585 Ionomycin Sigma Cat# I3909 brefeldin A Sigma Cat# B6542 human GM-CSF AcroBiosystems Cat# GMF-H8214 human GM-IL-4 AcroBiosystems Cat# IL-4-H4218 Mouse TNF-a DuoSet ELISA R&D Cat# DY410-05 Mouse IL-6 DouSet ELISA R&D Cat# DY406-05 Mouse IL-1b DouSet ELISA R&D Cat# DY401-05 Mouse IL-17 DouSet ELISA R&D Cat# DY421-05 Mouse IFN-g DouSet ELISA R&D Cat# DY485-05 Mouse IL-10 DouSet ELISA R&D Cat# DY-417-05 Mouse IL-12/P70 DouSet ELISA R&D Cat# DY-419-05 Mouse IL-23 DouSet ELISA R&D Cat# DY1887-05 Human IL-1b DouSet ELISA R&D Cat# DY201 Human IL-6 DouSet ELISA R&D Cat# DY206 Human IL-12/P70 ELISA KIT Invitrogen Cat# BMS238 Human TNF-a DouSet ELISA R&D Cat# DY210 Deposited data Microbiota 16S rRNA gene sequences (NCBI GenBank) This paper PRJNA718486 Oligonucleotides Mouse PXR F: 50-GACCTGCCTATTG AGGACCA-30 Sangon Biotech N/A Mouse PXRR: 50-TTCTGGAAGCCACCATTAGG-30 Sangon Biotech N/A Mouse a5b1 F: 50-AGCGACTGGAATCC TCAAGA-30 Sangon Biotech N/A Mouse a5b1 R: 50-TGCTGAGTCCTGT CACCTTG-30 Sangon Biotech N/A Mouse GR F: 50-AGGCCGCTCAGTGTTTTCTA-30 Sangon Biotech N/A Mouse GR R: 50-TACAGCTTCCACACGTCAGC-30 Sangon Biotech N/A Mouse TGR5 F: 50-TTCTCTCTGTCCG CGTGTTG-30 Sangon Biotech N/A Mouse TGR5 R: 50-GGTGCTGCCCAA TGAGATGA-30 Sangon Biotech N/A Mouse VDR F: 50-GATGAGGAGGTGC AGCGTAA-30 Sangon Biotech N/A (Continued on next page) Cell Reports 36, 109726, September 21, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse VDR R: 50-GTCGTAGGTCT TGTGGTGGG-30 Sangon Biotech N/A Mouse FXR F: 50-AGGGGATGAGC TGTGTGTTG 30 Sangon Biotech N/A Mouse FXR R: 50-ACACTGGATTTCAGTT AACAAACCT-30 Sangon Biotech N/A Mouse CAR F: 50-GGAGGACCAGA TCTCCCTTC-30 Sangon Biotech N/A Mouse CAR R: 50-CTCGTACTGGA ACCCTGCAT-30 Sangon Biotech N/A Mouse GAPDH F: 50-ACCCAGAA GACTGTGGATGG-30 Sangon Biotech N/A Mouse GAPDH R: 50-CACATTGGG GGTAGGAACAC-30 Sangon Biotech N/A Software and algorithms GraphPad Prism version 7.0 GraphPad Software https://www.graphpad.com UPARSE PMID: 23955772 http://www.drive5.com/usearch/ QIIME software PMID: 20383131 http://qiime.org/ SPSS version 22.0 SPSS https://www.ibm.com/products/ spss-statistics Flowjo version 10.0 Tree https://www.flowjo.com/

    Enzyme-linked Immunosorbent Assay:

    Article Title: Gut microbiota-mediated secondary bile acids regulate dendritic cells to attenuate autoimmune uveitis through TGR5 signaling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse CD4 APC eBioscience Cat# 17-0042-81; RRID:AB_469322 Anti-mouse IL-17a PE eBioscience Cat# 12-7177-81; RRID:AB_763582 Anti-mouse IFN-g PE-cy7 eBioscience Cat# 25-7311-82; RRID:AB_469680 Anti-mouse CD25 PE eBioscience Cat# 12-0251-81; RRID:AB_465606 Anti-mouse Foxp3 PE-cy7 eBioscience Cat# 25-5773-80; RRID:AB_891554 Anti-mouse CD11c APC BioLegend Cat# 117310; RRID:AB_313779 Anti-mouse CD40 FITC BioLegend Cat# 124607; RRID:AB_1134090 Anti-mouse MHCII PE eBioscience Cat# 12-5321-81; RRID:AB_465927 Anti-mouse CD80 PE eBioscience Cat# 12-0801-81; RRID:AB_465751 Anti-mouse CD86 FITC eBioscience Cat# 11-0860-82; RRID:AB_465145 Anti-human CD80 PE BioLegend Cat# 375410; RRID:AB_2890820 Anti-human CD86 PE-cy7 BioLegend Cat# 374209; RRID:AB_2728391 Anti-human HLA-DR PE-cy5.5 BioLegend Cat# 307629; RRID:AB_893575 Anti-human CD40 FITC BioLegend Cat# 334305; RRID:AB_1186056 Mouse CD4 microbeads Miltenyi Biotec Cat# 130-049-201; RRID:AB_2722753 Human CD14 microbeads Miltenyi Biotec Cat# 130-097-052; RRID:AB_2665482 Anti-TGR5 Abcam Cat# ab72608; RRID:AB_2112165 Anti-P65 Abcam Cat# ab16502; RRID:AB_443394 Anti-NF-kB p65 (Phospho Ser536) CST Cat# 3033T; RRID:AB_331284 Anti-Ikba Abcam Cat# ab32518; RRID:AB_733068 Anti-Ikba (phospho S36) Abcam Cat# ab133462; RRID:AB_2801653 Anti-CREB Abcam Cat# ab32515; RRID:AB_2292301 Anti-CREB(phosphor S133) Abcam Cat# ab32096; RRID:AB_731734 Anti-b-actin Abcam Cat# ab8227; RRID:AB_2305186 Experimental models: Organisms/strains C57BL/6j mice Jachson Lab N/A TGR5 / mice Viewsolid biotech N/A Bacterial and virus strains Clostridium scindens ATCC ATCC35704 Chemicals, peptides, and recombinant proteins IRBP651-670 Sangon Biotech N/A Mycobacterium tuberculosis strain H37Ra BD Cat# 231141 Bordetella pertussis toxin Sigma Cat# P7208 CFA Sigma Cat# F5881 QIAamp Fast DNA Stool Mini Kit QIAGEN Cat# 51604 Neomycin Sigma Cat# N6386 Ampicillin Sigma Cat# A5354 Metronidazole Sigma Cat# 16677 Vancomycin Sigma Cat# V2002 TRIzol Reagent Invitrogen Cat# 15596026 PrimeScript RT reagent Kit Takara Cat# DRR047A iTaq Universal SYBR Green Supermix BIO-RAD Cat# 1725124 (Continued on next page) e1 Cell Reports 36, 109726, September 21, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER mouse GM-CSF R&D Cat# 415-ML-50 mouse IL-4 R&D Cat# 404-ML-50 Lipopolysaccharide Sigma Cat# L2880 DCA Sigma Cat# L6250 INT-777 MedChemExpress Cat# HY-15677 Forskolin MedChemExpress Cat# HY-15371 IBMX MedChemExpress Cat# HY-12318 KH7 MedChemExpress Cat# HY-103194 MDL12330A Sigma Cat# 444200 PKI MedChemExpress Cat# HY-P0222 H89 MedChemExpress Cat# HY-15979 Foxp3 / Transcription Factor Staining Buffer Set Kit Invitrogen Cat# 00-5523-00 PMA Sigma Cat# P1585 Ionomycin Sigma Cat# I3909 brefeldin A Sigma Cat# B6542 human GM-CSF AcroBiosystems Cat# GMF-H8214 human GM-IL-4 AcroBiosystems Cat# IL-4-H4218 Mouse TNF-a DuoSet ELISA R&D Cat# DY410-05 Mouse IL-6 DouSet ELISA R&D Cat# DY406-05 Mouse IL-1b DouSet ELISA R&D Cat# DY401-05 Mouse IL-17 DouSet ELISA R&D Cat# DY421-05 Mouse IFN-g DouSet ELISA R&D Cat# DY485-05 Mouse IL-10 DouSet ELISA R&D Cat# DY-417-05 Mouse IL-12/P70 DouSet ELISA R&D Cat# DY-419-05 Mouse IL-23 DouSet ELISA R&D Cat# DY1887-05 Human IL-1b DouSet ELISA R&D Cat# DY201 Human IL-6 DouSet ELISA R&D Cat# DY206 Human IL-12/P70 ELISA KIT Invitrogen Cat# BMS238 Human TNF-a DouSet ELISA R&D Cat# DY210 Deposited data Microbiota 16S rRNA gene sequences (NCBI GenBank) This paper PRJNA718486 Oligonucleotides Mouse PXR F: 50-GACCTGCCTATTG AGGACCA-30 Sangon Biotech N/A Mouse PXRR: 50-TTCTGGAAGCCACCATTAGG-30 Sangon Biotech N/A Mouse a5b1 F: 50-AGCGACTGGAATCC TCAAGA-30 Sangon Biotech N/A Mouse a5b1 R: 50-TGCTGAGTCCTGT CACCTTG-30 Sangon Biotech N/A Mouse GR F: 50-AGGCCGCTCAGTGTTTTCTA-30 Sangon Biotech N/A Mouse GR R: 50-TACAGCTTCCACACGTCAGC-30 Sangon Biotech N/A Mouse TGR5 F: 50-TTCTCTCTGTCCG CGTGTTG-30 Sangon Biotech N/A Mouse TGR5 R: 50-GGTGCTGCCCAA TGAGATGA-30 Sangon Biotech N/A Mouse VDR F: 50-GATGAGGAGGTGC AGCGTAA-30 Sangon Biotech N/A (Continued on next page) Cell Reports 36, 109726, September 21, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse VDR R: 50-GTCGTAGGTCT TGTGGTGGG-30 Sangon Biotech N/A Mouse FXR F: 50-AGGGGATGAGC TGTGTGTTG 30 Sangon Biotech N/A Mouse FXR R: 50-ACACTGGATTTCAGTT AACAAACCT-30 Sangon Biotech N/A Mouse CAR F: 50-GGAGGACCAGA TCTCCCTTC-30 Sangon Biotech N/A Mouse CAR R: 50-CTCGTACTGGA ACCCTGCAT-30 Sangon Biotech N/A Mouse GAPDH F: 50-ACCCAGAA GACTGTGGATGG-30 Sangon Biotech N/A Mouse GAPDH R: 50-CACATTGGG GGTAGGAACAC-30 Sangon Biotech N/A Software and algorithms GraphPad Prism version 7.0 GraphPad Software https://www.graphpad.com UPARSE PMID: 23955772 http://www.drive5.com/usearch/ QIIME software PMID: 20383131 http://qiime.org/ SPSS version 22.0 SPSS https://www.ibm.com/products/ spss-statistics Flowjo version 10.0 Tree https://www.flowjo.com/



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    Figure 6. TGR5 signaling inhibits NF-kB activation via the cAMP-PKA pathway (A, C, and E) The phosphorylation levels of p65 and Ikba in LPS-primed BMDCs treated by INT-777 in the presence of forskolin <t>(A),</t> <t>KH7</t> and MDL12300A (C), and <t>PKI</t> and H89 (E) were assayed by western blot. (B, D, and F) The expression levels of IL-1b, IL-6, IL-12p70, and TNF-a in LPS-primed BMDCs treated by INT-777 in the presence of forskolin (B), KH7 and MDL12300A (D), PKI and H89 (F) were assayed by ELISA. n = 4 per group. Data are shown as mean ± SD. A one-way ANOVA was used. *p < 0.05, **p < 0.01; ns, not significant (p > 0.05). See also Figure S7.
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    Figure 6. TGR5 signaling inhibits NF-kB activation via the cAMP-PKA pathway (A, C, and E) The phosphorylation levels of p65 and Ikba in LPS-primed BMDCs treated by INT-777 in the presence of forskolin (A), KH7 and MDL12300A (C), and PKI and H89 (E) were assayed by western blot. (B, D, and F) The expression levels of IL-1b, IL-6, IL-12p70, and TNF-a in LPS-primed BMDCs treated by INT-777 in the presence of forskolin (B), KH7 and MDL12300A (D), PKI and H89 (F) were assayed by ELISA. n = 4 per group. Data are shown as mean ± SD. A one-way ANOVA was used. *p < 0.05, **p < 0.01; ns, not significant (p > 0.05). See also Figure S7.

    Journal: Cell reports

    Article Title: Gut microbiota-mediated secondary bile acids regulate dendritic cells to attenuate autoimmune uveitis through TGR5 signaling.

    doi: 10.1016/j.celrep.2021.109726

    Figure Lengend Snippet: Figure 6. TGR5 signaling inhibits NF-kB activation via the cAMP-PKA pathway (A, C, and E) The phosphorylation levels of p65 and Ikba in LPS-primed BMDCs treated by INT-777 in the presence of forskolin (A), KH7 and MDL12300A (C), and PKI and H89 (E) were assayed by western blot. (B, D, and F) The expression levels of IL-1b, IL-6, IL-12p70, and TNF-a in LPS-primed BMDCs treated by INT-777 in the presence of forskolin (B), KH7 and MDL12300A (D), PKI and H89 (F) were assayed by ELISA. n = 4 per group. Data are shown as mean ± SD. A one-way ANOVA was used. *p < 0.05, **p < 0.01; ns, not significant (p > 0.05). See also Figure S7.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER mouse GM-CSF R&D Cat# 415-ML-50 mouse IL-4 R&D Cat# 404-ML-50 Lipopolysaccharide Sigma Cat# L2880 DCA Sigma Cat# L6250 INT-777 MedChemExpress Cat# HY-15677 Forskolin MedChemExpress Cat# HY-15371 IBMX MedChemExpress Cat# HY-12318 KH7 MedChemExpress Cat# HY-103194 MDL12330A Sigma Cat# 444200 PKI MedChemExpress Cat# HY-P0222 H89 MedChemExpress Cat# HY-15979 Foxp3 / Transcription Factor Staining Buffer Set Kit Invitrogen Cat# 00-5523-00 PMA Sigma Cat# P1585 Ionomycin Sigma Cat# I3909 brefeldin A Sigma Cat# B6542 human GM-CSF AcroBiosystems Cat# GMF-H8214 human GM-IL-4 AcroBiosystems Cat# IL-4-H4218 Mouse TNF-a DuoSet ELISA R&D Cat# DY410-05 Mouse IL-6 DouSet ELISA R&D Cat# DY406-05 Mouse IL-1b DouSet ELISA R&D Cat# DY401-05 Mouse IL-17 DouSet ELISA R&D Cat# DY421-05 Mouse IFN-g DouSet ELISA R&D Cat# DY485-05 Mouse IL-10 DouSet ELISA R&D Cat# DY-417-05 Mouse IL-12/P70 DouSet ELISA R&D Cat# DY-419-05 Mouse IL-23 DouSet ELISA R&D Cat# DY1887-05 Human IL-1b DouSet ELISA R&D Cat# DY201 Human IL-6 DouSet ELISA R&D Cat# DY206 Human IL-12/P70 ELISA KIT Invitrogen Cat# BMS238 Human TNF-a DouSet ELISA R&D Cat# DY210 Deposited data Microbiota 16S rRNA gene sequences (NCBI GenBank) This paper PRJNA718486 Oligonucleotides Mouse PXR F: 50-GACCTGCCTATTG AGGACCA-30 Sangon Biotech N/A Mouse PXRR: 50-TTCTGGAAGCCACCATTAGG-30 Sangon Biotech N/A Mouse a5b1 F: 50-AGCGACTGGAATCC TCAAGA-30 Sangon Biotech N/A Mouse a5b1 R: 50-TGCTGAGTCCTGT CACCTTG-30 Sangon Biotech N/A Mouse GR F: 50-AGGCCGCTCAGTGTTTTCTA-30 Sangon Biotech N/A Mouse GR R: 50-TACAGCTTCCACACGTCAGC-30 Sangon Biotech N/A Mouse TGR5 F: 50-TTCTCTCTGTCCG CGTGTTG-30 Sangon Biotech N/A Mouse TGR5 R: 50-GGTGCTGCCCAA TGAGATGA-30 Sangon Biotech N/A Mouse VDR F: 50-GATGAGGAGGTGC AGCGTAA-30 Sangon Biotech N/A (Continued on next page) Cell Reports 36, 109726, September 21, 2021 e2

    Techniques: Activation Assay, Phospho-proteomics, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay